| Gosport Borough | VS | Salisbury FC | February 25 2020, 19:45 | The Aerial Direct Stadium |
| Harrow Borough FC | VS | Salisbury FC | February 29 2020, 15:00 | Earlsmead Stadium |
| Amesbury Town FC | VS | Salisbury FC | March 03 2020, 19:45 | Bonnymead Park |
| Salisbury FC | VS | Merthyr Town FC | March 07 2020, 15:00 | The Ray Mac |
| Truro City FC | VS | Salisbury FC | March 14 2020, 15:00 | Treyew Road |
| Salisbury FC | VS | Beaconsfield Town FC | March 17 2020, 19:45 | The Ray Mac |
| Salisbury FC | VS | Farnborough FC | March 21 2020, 15:00 | The Ray Mac |
| Beaconsfield Town FC | VS | Salisbury FC | March 28 2020, 15:00 | Holloways Park |
| Poole Town FC | VS | Salisbury FC | March 31 2020, 19:45 | Black Gold Stadium |
| Salisbury FC | VS | Hayes & Yeading United FC | April 04 2020, 15:00 | The Ray Mac |
| Salisbury FC | VS | Weston-super-Mare AFC | April 11 2020, 15:00 | The Ray Mac |
| Wimborne Town FC | VS | Salisbury FC | April 13 2020, 15:00 | The Cuthbury |
| Salisbury FC | VS | Walton Casuals FC | April 18 2020, 15:00 | The Ray Mac |
| Blackfield & Langley FC | VS | Salisbury FC | April 25 2020, 15:00 | Gang Warily |